Studies on Hog Intrinsic Factor

Studies on the mechanism of cobalamin binding to hog intrinsic factor.

Cobalamin binding to hog intrinsic factor has been studied by affinity chromatography, and the cobalt atom of the cobalamin tested was found to be no more than 5 A from the surface of the protein. A comparative study of the binding of the c carboxylic acid derivative versus the c amide at C, of the B-pyrrole ring indicates that a change in charge at this position of the corrin ring has little e...

Hog Intrinsic Factor I. ISOLATION OF VITAMIN Bn-BINDING FRACTIONS FROM HOG PYLORUS”

Intrinsic factor and another high capacity vitamin Blzbinding protein devoid of intrinsic factor activity were isolated from hog pyloric mucosa. Both components were isolated as their vitamin Blz complexes and in noncomplexed form. All four preparations are glycoproteins and appear to be homogeneous as judged by starch gel electrophoresis, velocity sedimentation analysis, column chromatography,...

Hog intrinsic factor. I. Isolation of vitamin B12-binding fractions from hog pylorus.

Intrinsic factor and another high capacity vitamin Blzbinding protein devoid of intrinsic factor activity were isolated from hog pyloric mucosa. Both components were isolated as their vitamin Blz complexes and in noncomplexed form. All four preparations are glycoproteins and appear to be homogeneous as judged by starch gel electrophoresis, velocity sedimentation analysis, column chromatography,...

Studies on antibody to intrinsic factor.

Sera from a group of 79 patients with pernicious anemia were studied for the presence of antibody to intrinsic factor. Two general types of antibody activity were found, and it was possible to distinguish three groups of pernicious anemia sera on the basis of their content of these types. Type I antibody blocks the binding of radioactive vitamin B(12) to intrinsic factor when added to intrinsic...

Studies on Secretion of Gastric Intrinsic Factor in Man.